Sanger Sequencing

Sanger Sequencing
Overnight Turnaround, Starting at Just $3

Overview
Accelerate your research with Quintara's Sanger Sequencing services. We deliver accurate and reliable Sanger DNA sequencing results with industry-leading overnight turnaround, while providing 90+ free universal sequencing primers to streamline your workflow and reduce overall sanger sequencing cost. Our services support a wide range of applications, including plasmid verification, mutation analysis, PCR product sequencing, clone confirmation, and colony sequencing.

Designed for efficiency and consistency, Quintara's sanger sequencing solutions combine fast turnaround, competitive pricing, and expertise in difficult-to-sequence regions to help academic, biotechnology, and pharmaceutical laboratories move projects forward without sacrificing data quality.

Back To School Promotion Last Call

Offer:

Sanger Sequencing (Premixed): 25% OFF, only $3.00/sample after savings

Sanger Sequencing (non-Premixed): 20% OFF, only $4.00/sample after savings

Terms and Conditions:

?The promotional pricing applies to all qualifying Sanger Sequencing orders valid Sep 1 through Oct 31, based on ET.

?The discount will be applied automatically when you place your order through the QuinGo online portal (Promo Code: 26BACKSanger).

?Quintara Biosciences reserves all rights to this promotion.

Why Choose Quintara Sanger Sequencing Services?

Overnight Turnaround 1000+ Free Drop Boxes

Easy Online Ordering

90+ Free Universal Sequencing Primers

Expertise in Difficult Template Sequencing

Sanger Sequencing Services
Name Description Turnaround* Price
Sanger Sequencing Samples can be mixed or non-mixed with primers Overnight Starting from
$4 $3 +
Yeast Colony Sequencing Direct colony sequencing Overnight Starting from $15
Value-Added Services ? PCR Amplification & Clean-Up: We'll amplify and purify your PCR reactions from raw product. / Starting from $1
(We provide specialized purification and preparation services tailored to your starting material.) ? RCA Clean-Up: We specialize in purifying Rolling Circle Amplification products.
? Plasmid Mini-preps: Get pure plasmid DNA directly from bacterial cultures.
* Your project clock starts once we receive your samples.

* Our services accept a range of sample types, including unpurified PCR products, Genomic DNA, RCA products, and bacterial cultures.

+ Promotion Price

What Is Sanger Sequencing?

Sanger sequencing, also known as Sanger DNA sequencing or the chain-termination method, is a foundational technique for determining the exact nucleotide sequence (A, T, C, G) in DNA fragments. Developed by Frederick Sanger in 1977, it remains the gold standard for high-accuracy, short-to-medium length DNA sequencing.

The method relies on controlled termination of DNA synthesis using dideoxynucleotides (ddNTPs). As DNA polymerase builds new strands, ddNTPs randomly stop the reaction at specific bases, generating fragments of varying lengths. These fragments are separated by capillary electrophoresis and read as a clear, accurate sequence with typical read lengths up to 1,000 bases and Phred20 quality.

At Quintara Bio, we leverage this proven technology to deliver reliable results with overnight turnaround, competitive Sanger sequencing cost, and specialized expertise in difficult templates.

How Does Sanger Sequencing Work?

Sanger sequencing works by combining DNA polymerase, sequencing primers, standard deoxynucleotides (dNTPs), and fluorescently labeled dideoxynucleotides (ddNTPs) during DNA synthesis. As DNA strands are extended, ddNTPs terminate strand elongation at specific bases, generating DNA fragments of different lengths.

These fragments are then separated by capillary electrophoresis, allowing the DNA sequence to be accurately determined from the resulting fluorescent signal patterns. PCR amplification is commonly used before Sanger DNA sequencing to generate sufficient target DNA for analysis.

Sample Submission Guideline
Sample tube type: please use 8-strip tubes. If the number of samples is large, a 96-well plate may be used. Please clearly indicate whether the samples are arranged by row or by column.

Primer tube type: please use individual microfuge tubes. (Primers should be 18–25 bp in length, with a GC content of 40–60% and a melting temperature (Tm) of 55–60 °C. Avoid secondary structures or primer dimers. The recommended primer concentration is 5 ?M.) You may also use Quintara free universal primers.

Submit the order through the QuinGo online portal and print the packing slip and place it in the sample bag together with the samples.

Send the samples by mail or drop them into the drop box before the cutoff time.

In?Stock Products?
Product Description Size Catalog No.
QuinDye™ Terminator QuinDye™ Terminator Kit provides premixed reagents for Sanger sequencing reactions. The kit includes Dye Terminator and a 5x buffer. 24 reactions CPR00001
500 reactions CPR00002
1,000 reactions CPR00003
5,000 reactions CPR00004